Techniques in Molecular Biology
Learn DNA extraction, Electrophoresis, Blotting, Primer designing, PCR, DNA sequencing
3.63 (24 reviews)
65
students
1 hour
content
Sep 2023
last update
$15.99
regular price
What you will learn
How DNA extraction is performed, components roles, what's the use of extracted DNA, DNA quantification
Electrophoresis, Agarose gel electrophoresis, Principle, How to make agarose gel, role of each component, DNA recovery
SDS- PAGE, role of stacking and seperating gel, role of each component, Buffer mechanism
Nucleic Acid Hybridization, Probing
Southern, Northern and Western blotting, their examples and applications, difference between different blotting techniques
PCR- Principle, procedure, analysis of PCR products, applications of PCR
Primers designing, parameters of primer designing, Tm- melting temperature
DNA sequencing- Difference between PCR and sequencing, detection, capillary electrophoresis and applications of DNA sequencing.
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4750164
udemy ID
24/06/2022
course created date
30/06/2022
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