CRISPR Cas System: Applications in Gene Editing and Beyond
CRISPR in gene editing, gene regulation, ChIP, live-cell imaging, diagnosis etc. | Genetics | Immunology | Biotechnology
4.35 (348 reviews)
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6 hours
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Mar 2023
last update
$74.99
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What you will learn
Introductory overview of basic concepts of Molecular Biology
What is gene editing?
Gene editing tools: Zinc Finger Nucleases (ZFNs) and TALENs and their limitations
DNA repair by Homology Directed Repair (HDR) pathway and Non Homologous End joining (NHEJ) repair
Introduction to CRISPR-Cas system and how it can be used as a gene editing tool?
Components, types and mechanism of CRISPR-Cas system
Protospacer Adjacent Motifs or PAMs
Off target activity of SpCas9 nuclease
Variants of Cas9 nuclease: Cas9 nickase, dead Cas9 (dCas9) and FokI fused catalytically inactive Cas9,
Cas9 nucleases with novel PAM specificities
High Fidelity SpCas9
Cas9 orthologs: SaCas9, NmCas9, CjCas9
Viral vectors for CRISPR delivery: Retroviral vectors, Adenoviral vectors, AAV vectors, etc.
Prime editing: its components, mechanism, applications, advantages and limitations
CRISPR-mediated Base editing: its components, mechanism, applications, advantages and limitations
CRISPR activation or CRISPRa
CRISPR interference or CRISPRi
Epigenetic editing with dCas9
CRISPR-mediated Chromatin Immunoprecipitation (ChIP)
Fluorescence In Situ Hybridization (FISH)
CRISPR-mediated live-cell imaging of genomic loci
Cas12 and Cas13 enzymes for diagnosing infectious diseases
Sherlock CRISPR-Cas13-based diagnostic kit
DETECTOR: CRISPR-Cas12-based diagnostic kit
FELUDA: CRISPR-Cas19-based diagnostic kit
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3889916
udemy ID
04/03/2021
course created date
24/08/2022
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